Thông tin luận án NCS Vũ Thị Huyền Trang – Ngành Công nghệ Sinh học
+ Nghiên cứu sinh: Vũ Thị Huyền Trang
+ Tên luận án: MOLECULAR IDENTIFICATION OF VIETNAM PAPHIOPEDILUM ORCHIDS USING DNA SEQUENCES.
+ Người hướng dẫn khoa học: PGS.TS. Lê Thị Lý – PGS.TS. Trần Hoàng Dũng
+ Những đóng góp học thuật của luận án:
1. Constructing the sequence datasets of ITS, matK and trnL regions for species of Paphiopedilum population of Vietnam. All data from the study was upload to GenBank library for extensive research. Our results were also given back to scientific Institutes which will contribute to the conservation and control of the illegal trade of Paphiopedilum species in Vietnam.
2. Sequences of the natural hybrid endemic species Paphiopedilum x dalatense of Vietnam were first reported in GenBank.
3. Proposing the use of the combination sequence matK + ITS for identification of Paphiopedilum in Vietnam and on the world.
4. Developing new effective primers for amplification of the universal locus matK, and the trnL fragment.
5. Providing the detailed vegetative artificial key for quick identification of Paphiopedilum in practical as a support tool in the combination with molecular tool.
6. Successful identifying 17 Vietnam Paphiopedilum species using DNA sequence and 20 species using the combination of vegetative and DNA features.
7. Giving the comparison among different bioinformatics tools and different bioinformatics analyses in barcoding technique and suggesting the proper tools for practical use.
8. The complete chloroplast genome of the species Paphiopedilum delenatii was first sequenced, assembled, annotated, and registered with GenBank. Character information from this platome was recommended to be effectively used for identification of closely related taxa and for developing many other identification markers.
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